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p p65 rabbit ab  (Bioss)


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    Structured Review

    Bioss p p65 rabbit ab
    The mRNA expression of ITGAV, FAK, PLC, PKC, <t>p65,</t> ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.
    P P65 Rabbit Ab, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+p+p65/NFKB+p65(Ser536)+Polyclonal+Antibody/pmc13125192-134-0-4
    Average 95 stars, based on 151 article reviews
    p p65 rabbit ab - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor"

    Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

    Journal: Poultry Science

    doi: 10.1016/j.psj.2026.106922

    The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.
    Figure Legend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

    Techniques Used: Expressing

    The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.
    Figure Legend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

    Techniques Used: Activity Assay

    Related Articles

    Incubation:

    Article Title: Peptide-directed interference of PD-1/PD-L1 binding increases B lymphocyte function after infectious bursal disease viral infection.
    Article Snippet: .. The membranes containing protein samples were blocked with 5% fat-free milk for 2 h and incubated overnight with primary antibodies: anti-b-actin (1:3000; T40104S, Abmart, Shanghai, China), anti-IBDV-VP2 (1:1,000; M032148S, Abmart, Shanghai, China), anti-Bax (1:000; 50599-2-Ig, Proteintech, Wuhan, China), anti-caspase-3 (1:1000; 66470-2-Ig, Proteintech, Wuhan, China), antiBcl-2 (1:1,000; MN50198, Abmart, Shanghai, China), anti-caspase-9 (1:1000; bs-8502R, Bioss, Beijing, China), anti-IkB-a (1:1,000; T55026, Abmart, Shanghai, China), anti-P65 (1:1000; P76367R1S, Abmart, Shanghai, China), anti-p-P65 (1:1000; bs-0982R, Bioss, Beijing, China), anti-p-PI3K (1:1000; TA4371, Abmart, Shanghai, China), anti-PI3K (1:1000; TA5121, Abmart, Shanghai, China), anti-p-AKT (1:1000; TA0016, Abmart, Shanghai, China), and anti-AKT (1:1000; PA1036, Abmart, Shanghai, China). ..

    Article Title: tRF-AspGTC Promotes Intracranial Aneurysm Formation by Controlling TRIM29-Mediated Galectin-3 Ubiquitination
    Article Snippet: .. After blocking, the membrane was incubated with the primary antibody overnight at 4 °C: anti-MHC (K002095P, Solarbio, China), anti-CNN1 (abs171608, Absin, China), anti-α-SMA (BM0002, Boster, China), anti-galectin-3 (60207-1-Ig, Proteintech, China), anti-TRIM29 (17542-1-AP, Proteintech, China), anti-MMP9 (BM4089, Boster, China), anti-MMP2 (A00286-2, Boster, China), anti-ubiquitin (#3936, Cell Signaling Technology, America), anti-TLR4 (66350-1-Ig, Proteintech, China), anti-MyD88 (23230-1-AP, Proteintech, China), anti-IκBα (abs131168, Absin, China), anti-phosphorylated inhibitor of nuclear factor kappa-B alpha (abs172314, Absin, China), anti-P65 (BF8005, Affinity, China), anti-p-P65 (bs-0271R, BIOSS, China), and anti-β-actin (66009-1-Ig, Proteintech, China). .. After washing, membranes were incubated with horseradish peroxidase-conjugated secondary antibody for 1 h. Signals were detected using a chemiluminescence imaging system (Millipore, USA) with the lumiQ Universal ECL Substrate (SB-WB012, Share-bio, Shanghai) and quantified using the ImageJ software, normalized to β-actin levels.

    Article Title: Coptis chinensis extracellular vesicles loaded with CA1-siRNA promote endothelial repair and stent restenosis therapy by regulating the PADI2 and NF-κB pathway.
    Article Snippet: .. After sealing, the membrane was incubated overnight with primary antibodies at 4 °C: anti-CA1 (13198-2-AP, Proteintech, China), anti-IL-6 (16806-1-AP, Proteintech, China), anti-IL-1β (K002095P, Solarbio, China), antiTNF-α (K002095P, Solarbio, China), anti-alpha SMA (BM0002, Boster, China), anti-MMP9 (BM4089, Boster, China), anti-IκBα (abs131168, absinthe, China), antiIκBα phosphorylation (abs172314, absinthe, China), anti-p65 (BF8005, Affinity, China), anti-p-p65 (bs-0271R, BIOSS, China), and anti-β-actin (66009-1-Ig, Proteintech, China). .. After washing, incubate the membrane with horseradish peroxidase conjugated secondary antibody for 1 h. The signals were detected using a chemiluminescence imaging system (Millipore, USA) and LumiQ universal ECL substrate (SB-WB012, Solarbio, Shanghai), and quantified using ImageJ software, normalized to β-actin levels.

    Article Title: An orally-administered nanotherapeutics with gold nanospheres supplying for rheumatoid arthritis therapy by re-shaping gut microbial tryptophan metabolism
    Article Snippet: .. Paw tissue sections were blocked with 5% BSA and incubated overnight at 4 °C with primary antibodies: anti-PTEN (Cat#: 60,300–1, Proteintech, 1:200), anti-p-p65 (Cat#: bs-3543R, Bioss, 1:200). ..

    Article Title: Peptide-directed interference of PD-1/PD-L1 binding increases B lymphocyte function after infectious bursal disease viral infection
    Article Snippet: .. The membranes containing protein samples were blocked with 5% fat-free milk for 2 h and incubated overnight with primary antibodies: anti-β-actin (1:3000; T40104S, Abmart, Shanghai, China), anti-IBDV-VP2 (1:1,000; M032148S, Abmart, Shanghai, China), anti-Bax (1:000; 50599-2-Ig, Proteintech, Wuhan, China), anti-caspase-3 (1:1000; 66470-2-Ig, Proteintech, Wuhan, China), anti-Bcl-2 (1:1,000; MN50198, Abmart, Shanghai, China), anti-caspase-9 (1:1000; bs-8502R, Bioss, Beijing, China), anti-IκB-α (1:1,000; T55026, Abmart, Shanghai, China), anti-P65 (1:1000; P76367R1S, Abmart, Shanghai, China), anti-p-P65 (1:1000; bs-0982R, Bioss, Beijing, China), anti-p-PI3K (1:1000; TA4371, Abmart, Shanghai, China), anti-PI3K (1:1000; TA5121, Abmart, Shanghai, China), anti-p-AKT (1:1000; TA0016, Abmart, Shanghai, China), and anti-AKT (1:1000; PA1036, Abmart, Shanghai, China). ..

    Article Title: Coptis chinensis extracellular vesicles loaded with CA1-siRNA promote endothelial repair and stent restenosis therapy by regulating the PADI2 and NF-κB pathway
    Article Snippet: .. After sealing, the membrane was incubated overnight with primary antibodies at 4 °C: anti-CA1 (13198-2-AP, Proteintech, China), anti-IL-6 (16806-1-AP, Proteintech, China), anti-IL-1β (K002095P, Solarbio, China), anti-TNF-α (K002095P, Solarbio, China), anti-alpha SMA (BM0002, Boster, China), anti-MMP9 (BM4089, Boster, China), anti-IκBα (abs131168, absinthe, China), anti- IκBα phosphorylation (abs172314, absinthe, China), anti-p65 (BF8005, Affinity, China), anti-p-p65 (bs-0271R, BIOSS, China), and anti-β-actin (66009-1-Ig, Proteintech, China). .. After washing, incubate the membrane with horseradish peroxidase conjugated secondary antibody for 1 h. The signals were detected using a chemiluminescence imaging system (Millipore, USA) and LumiQ universal ECL substrate (SB-WB012, Solarbio, Shanghai), and quantified using ImageJ software, normalized to β-actin levels.

    Article Title: tRF-AspGTC promotes intracranial aneurysm formation by controlling TRIM29-mediated galectin-3 ubiquitination
    Article Snippet: .. After blocking, the membrane was incubated with the primary antibody overnight at 4°C: anti-MHC (K002095P, Solarbio, China), anti-CNN1 (abs171608, Absin, China), anti-α-SMA (BM0002, Boster, China), anti-galectin-3 (60207-1-Ig, Proteintech, China), anti-TRIM29 (17542-1-AP, Proteintech, China), anti-MMP9 (BM4089, Boster, China), anti-MMP2 (A00286-2, Boster, China), anti-ubiquitin (#3936, Cell Signaling Technology, America), anti-TLR4 (66350-1-Ig, Proteintech, China), anti-MyD88 (23230-1-AP, Proteintech, China), anti-IκBα (abs131168, Absin, China), anti-p-IκBα (abs172314, Absin, China), anti-P65 (BF8005, Affinity, China), anti-p-P65 (bs-0271R, BIOSS, China), anti-β-actin (66009-1-Ig, Proteintech, China). ..

    Blocking Assay:

    Article Title: tRF-AspGTC Promotes Intracranial Aneurysm Formation by Controlling TRIM29-Mediated Galectin-3 Ubiquitination
    Article Snippet: .. After blocking, the membrane was incubated with the primary antibody overnight at 4 °C: anti-MHC (K002095P, Solarbio, China), anti-CNN1 (abs171608, Absin, China), anti-α-SMA (BM0002, Boster, China), anti-galectin-3 (60207-1-Ig, Proteintech, China), anti-TRIM29 (17542-1-AP, Proteintech, China), anti-MMP9 (BM4089, Boster, China), anti-MMP2 (A00286-2, Boster, China), anti-ubiquitin (#3936, Cell Signaling Technology, America), anti-TLR4 (66350-1-Ig, Proteintech, China), anti-MyD88 (23230-1-AP, Proteintech, China), anti-IκBα (abs131168, Absin, China), anti-phosphorylated inhibitor of nuclear factor kappa-B alpha (abs172314, Absin, China), anti-P65 (BF8005, Affinity, China), anti-p-P65 (bs-0271R, BIOSS, China), and anti-β-actin (66009-1-Ig, Proteintech, China). .. After washing, membranes were incubated with horseradish peroxidase-conjugated secondary antibody for 1 h. Signals were detected using a chemiluminescence imaging system (Millipore, USA) with the lumiQ Universal ECL Substrate (SB-WB012, Share-bio, Shanghai) and quantified using the ImageJ software, normalized to β-actin levels.

    Article Title: tRF-AspGTC promotes intracranial aneurysm formation by controlling TRIM29-mediated galectin-3 ubiquitination
    Article Snippet: .. After blocking, the membrane was incubated with the primary antibody overnight at 4°C: anti-MHC (K002095P, Solarbio, China), anti-CNN1 (abs171608, Absin, China), anti-α-SMA (BM0002, Boster, China), anti-galectin-3 (60207-1-Ig, Proteintech, China), anti-TRIM29 (17542-1-AP, Proteintech, China), anti-MMP9 (BM4089, Boster, China), anti-MMP2 (A00286-2, Boster, China), anti-ubiquitin (#3936, Cell Signaling Technology, America), anti-TLR4 (66350-1-Ig, Proteintech, China), anti-MyD88 (23230-1-AP, Proteintech, China), anti-IκBα (abs131168, Absin, China), anti-p-IκBα (abs172314, Absin, China), anti-P65 (BF8005, Affinity, China), anti-p-P65 (bs-0271R, BIOSS, China), anti-β-actin (66009-1-Ig, Proteintech, China). ..

    Membrane:

    Article Title: tRF-AspGTC Promotes Intracranial Aneurysm Formation by Controlling TRIM29-Mediated Galectin-3 Ubiquitination
    Article Snippet: .. After blocking, the membrane was incubated with the primary antibody overnight at 4 °C: anti-MHC (K002095P, Solarbio, China), anti-CNN1 (abs171608, Absin, China), anti-α-SMA (BM0002, Boster, China), anti-galectin-3 (60207-1-Ig, Proteintech, China), anti-TRIM29 (17542-1-AP, Proteintech, China), anti-MMP9 (BM4089, Boster, China), anti-MMP2 (A00286-2, Boster, China), anti-ubiquitin (#3936, Cell Signaling Technology, America), anti-TLR4 (66350-1-Ig, Proteintech, China), anti-MyD88 (23230-1-AP, Proteintech, China), anti-IκBα (abs131168, Absin, China), anti-phosphorylated inhibitor of nuclear factor kappa-B alpha (abs172314, Absin, China), anti-P65 (BF8005, Affinity, China), anti-p-P65 (bs-0271R, BIOSS, China), and anti-β-actin (66009-1-Ig, Proteintech, China). .. After washing, membranes were incubated with horseradish peroxidase-conjugated secondary antibody for 1 h. Signals were detected using a chemiluminescence imaging system (Millipore, USA) with the lumiQ Universal ECL Substrate (SB-WB012, Share-bio, Shanghai) and quantified using the ImageJ software, normalized to β-actin levels.

    Article Title: Coptis chinensis extracellular vesicles loaded with CA1-siRNA promote endothelial repair and stent restenosis therapy by regulating the PADI2 and NF-κB pathway.
    Article Snippet: .. After sealing, the membrane was incubated overnight with primary antibodies at 4 °C: anti-CA1 (13198-2-AP, Proteintech, China), anti-IL-6 (16806-1-AP, Proteintech, China), anti-IL-1β (K002095P, Solarbio, China), antiTNF-α (K002095P, Solarbio, China), anti-alpha SMA (BM0002, Boster, China), anti-MMP9 (BM4089, Boster, China), anti-IκBα (abs131168, absinthe, China), antiIκBα phosphorylation (abs172314, absinthe, China), anti-p65 (BF8005, Affinity, China), anti-p-p65 (bs-0271R, BIOSS, China), and anti-β-actin (66009-1-Ig, Proteintech, China). .. After washing, incubate the membrane with horseradish peroxidase conjugated secondary antibody for 1 h. The signals were detected using a chemiluminescence imaging system (Millipore, USA) and LumiQ universal ECL substrate (SB-WB012, Solarbio, Shanghai), and quantified using ImageJ software, normalized to β-actin levels.

    Article Title: Coptis chinensis extracellular vesicles loaded with CA1-siRNA promote endothelial repair and stent restenosis therapy by regulating the PADI2 and NF-κB pathway
    Article Snippet: .. After sealing, the membrane was incubated overnight with primary antibodies at 4 °C: anti-CA1 (13198-2-AP, Proteintech, China), anti-IL-6 (16806-1-AP, Proteintech, China), anti-IL-1β (K002095P, Solarbio, China), anti-TNF-α (K002095P, Solarbio, China), anti-alpha SMA (BM0002, Boster, China), anti-MMP9 (BM4089, Boster, China), anti-IκBα (abs131168, absinthe, China), anti- IκBα phosphorylation (abs172314, absinthe, China), anti-p65 (BF8005, Affinity, China), anti-p-p65 (bs-0271R, BIOSS, China), and anti-β-actin (66009-1-Ig, Proteintech, China). .. After washing, incubate the membrane with horseradish peroxidase conjugated secondary antibody for 1 h. The signals were detected using a chemiluminescence imaging system (Millipore, USA) and LumiQ universal ECL substrate (SB-WB012, Solarbio, Shanghai), and quantified using ImageJ software, normalized to β-actin levels.

    Article Title: tRF-AspGTC promotes intracranial aneurysm formation by controlling TRIM29-mediated galectin-3 ubiquitination
    Article Snippet: .. After blocking, the membrane was incubated with the primary antibody overnight at 4°C: anti-MHC (K002095P, Solarbio, China), anti-CNN1 (abs171608, Absin, China), anti-α-SMA (BM0002, Boster, China), anti-galectin-3 (60207-1-Ig, Proteintech, China), anti-TRIM29 (17542-1-AP, Proteintech, China), anti-MMP9 (BM4089, Boster, China), anti-MMP2 (A00286-2, Boster, China), anti-ubiquitin (#3936, Cell Signaling Technology, America), anti-TLR4 (66350-1-Ig, Proteintech, China), anti-MyD88 (23230-1-AP, Proteintech, China), anti-IκBα (abs131168, Absin, China), anti-p-IκBα (abs172314, Absin, China), anti-P65 (BF8005, Affinity, China), anti-p-P65 (bs-0271R, BIOSS, China), anti-β-actin (66009-1-Ig, Proteintech, China). ..

    Phospho-proteomics:

    Article Title: Coptis chinensis extracellular vesicles loaded with CA1-siRNA promote endothelial repair and stent restenosis therapy by regulating the PADI2 and NF-κB pathway.
    Article Snippet: .. After sealing, the membrane was incubated overnight with primary antibodies at 4 °C: anti-CA1 (13198-2-AP, Proteintech, China), anti-IL-6 (16806-1-AP, Proteintech, China), anti-IL-1β (K002095P, Solarbio, China), antiTNF-α (K002095P, Solarbio, China), anti-alpha SMA (BM0002, Boster, China), anti-MMP9 (BM4089, Boster, China), anti-IκBα (abs131168, absinthe, China), antiIκBα phosphorylation (abs172314, absinthe, China), anti-p65 (BF8005, Affinity, China), anti-p-p65 (bs-0271R, BIOSS, China), and anti-β-actin (66009-1-Ig, Proteintech, China). .. After washing, incubate the membrane with horseradish peroxidase conjugated secondary antibody for 1 h. The signals were detected using a chemiluminescence imaging system (Millipore, USA) and LumiQ universal ECL substrate (SB-WB012, Solarbio, Shanghai), and quantified using ImageJ software, normalized to β-actin levels.

    Article Title: Coptis chinensis extracellular vesicles loaded with CA1-siRNA promote endothelial repair and stent restenosis therapy by regulating the PADI2 and NF-κB pathway
    Article Snippet: .. After sealing, the membrane was incubated overnight with primary antibodies at 4 °C: anti-CA1 (13198-2-AP, Proteintech, China), anti-IL-6 (16806-1-AP, Proteintech, China), anti-IL-1β (K002095P, Solarbio, China), anti-TNF-α (K002095P, Solarbio, China), anti-alpha SMA (BM0002, Boster, China), anti-MMP9 (BM4089, Boster, China), anti-IκBα (abs131168, absinthe, China), anti- IκBα phosphorylation (abs172314, absinthe, China), anti-p65 (BF8005, Affinity, China), anti-p-p65 (bs-0271R, BIOSS, China), and anti-β-actin (66009-1-Ig, Proteintech, China). .. After washing, incubate the membrane with horseradish peroxidase conjugated secondary antibody for 1 h. The signals were detected using a chemiluminescence imaging system (Millipore, USA) and LumiQ universal ECL substrate (SB-WB012, Solarbio, Shanghai), and quantified using ImageJ software, normalized to β-actin levels.

    Luciferase:

    Article Title: ARHGEF18 can promote BVDV NS5B activation of the host NF-κB signaling pathway by combining with the NS5B-palm domain.
    Article Snippet: Rho guanine nucleotide exchange factor 18 (ARHGEF18) is a member of the Rho guanine nucleotide exchange factor (RhoGEF) family.. RhoGEF plays an important role in the occurrence of tumors and neurological diseases; however, its involvement in host cell resistance against pathogenic microorganisms is mostly unknown.. Herein, we report that bovine viral diarrhea virus (BVDV) nonstructural protein 5B (NS5B) can activate the nuclear factor kappa B (NF-κB) signaling pathway to induce an immune response.



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    Image Search Results


    The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

    Journal: Poultry Science

    Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

    doi: 10.1016/j.psj.2026.106922

    Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

    Article Snippet: p-p65 Rabbit Ab , Bioss , bs-0982R , 1: 1500.

    Techniques: Expressing

    The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

    Journal: Poultry Science

    Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

    doi: 10.1016/j.psj.2026.106922

    Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

    Article Snippet: p-p65 Rabbit Ab , Bioss , bs-0982R , 1: 1500.

    Techniques: Activity Assay

    Potential therapeutic mechanism of Cryogel@USPB in wounds. (A) The relative mRNA expression of STING1 and interferon alpha after the treatment of Cryogel@USPB (n = 3). (B) Immunofluorescent staining of F4/80 (green), STING (red), and DAPI (blue) on day7 in diabetic wounds. (C) Immunofluorescent staining of F4/80 (green), STING (red), and DAPI (blue) on day7 in deep second-degree burn wounds. (D) Expression of STING, p-STING, cGAS, IRF3, p-IRF3, p65, P-p65 in three individual skin wound tissues of diabetic mice in the Cryogel@USPB and control group. (E) Expression of STING, p-STING, cGAS, IRF3, P-IRF3, p65, p-p65 in three individual skin tissues of deep second-degree burn wounds in the Cryogel@USPB and control group. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Ultrasmall Prussian blue–integrated cryogel for enhanced ROS scavenging and immunomodulation via cGAS–STING inhibition in wound healing

    doi: 10.1016/j.mtbio.2026.103056

    Figure Lengend Snippet: Potential therapeutic mechanism of Cryogel@USPB in wounds. (A) The relative mRNA expression of STING1 and interferon alpha after the treatment of Cryogel@USPB (n = 3). (B) Immunofluorescent staining of F4/80 (green), STING (red), and DAPI (blue) on day7 in diabetic wounds. (C) Immunofluorescent staining of F4/80 (green), STING (red), and DAPI (blue) on day7 in deep second-degree burn wounds. (D) Expression of STING, p-STING, cGAS, IRF3, p-IRF3, p65, P-p65 in three individual skin wound tissues of diabetic mice in the Cryogel@USPB and control group. (E) Expression of STING, p-STING, cGAS, IRF3, P-IRF3, p65, p-p65 in three individual skin tissues of deep second-degree burn wounds in the Cryogel@USPB and control group. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: The primary antibodies were: cGAS (1:1000, Proteintech, USA), STING (1:1000, Proteintech, USA), p-STING (1:1000, Proteintech, USA), IRF3 (1:1000, CST, USA), p-IRF3(1:1000, CST, USA), p65(1:1000, CST, USA ) , p-p65(1:1000, CST, USA ) , GADPH (1:5000, CST, USA) and β-actin (1:5000, CST, USA).

    Techniques: Expressing, Staining, Control

    CAP upregulates PRKCQ and activates NF-κB signaling in iMC23 cells iMC23 cells were treated with CAP for 0, 40, 60, or 80 s and then cultured for 48 h. (A) RT-qPCR analysis of PRKCQ mRNA levels. (B) Representative western blotting images showing PRKCQ and p-NF-κB p65 protein expression. (C) Semi-quantitative results of western blotting. (D) Cellular immunofluorescence staining for evaluating p-NF-κB p65 distribution in iMC23 cells. Scale bars, 50 μm. n = 3 biological replicates. Data are represented as mean ± standard deviation. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc analysis. ∗∗∗ p < 0.001. ns, no significance.

    Journal: iScience

    Article Title: Cold atmospheric plasma (CAP) stimulates melanoblast-to-melanocyte differentiation via PRKCQ upregulation and NF-κB activation

    doi: 10.1016/j.isci.2026.115609

    Figure Lengend Snippet: CAP upregulates PRKCQ and activates NF-κB signaling in iMC23 cells iMC23 cells were treated with CAP for 0, 40, 60, or 80 s and then cultured for 48 h. (A) RT-qPCR analysis of PRKCQ mRNA levels. (B) Representative western blotting images showing PRKCQ and p-NF-κB p65 protein expression. (C) Semi-quantitative results of western blotting. (D) Cellular immunofluorescence staining for evaluating p-NF-κB p65 distribution in iMC23 cells. Scale bars, 50 μm. n = 3 biological replicates. Data are represented as mean ± standard deviation. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc analysis. ∗∗∗ p < 0.001. ns, no significance.

    Article Snippet: After blocking with 5% skim milk in 0.1% TBST, the membrane was subjected to overnight incubation with the following primary antibodies: anti-TRP-2 (ab221144, 1:1000, Abcam), anti-TYRP1 (ab235447, 1:1000, Abcam), anti-TYR (A1254, 1:500, ABclonal), anti-MITF (ab303530, 1:1000, Abcam), anti-protein kinase C Theta (PRKCQ; 1:1000, ab302891, Abcam), anti-p65 (1:5000, A19653, ABclonal), anti-p-p65 (1:500, AP0124, ABclonal), and anti-β-actin (1:80000, AC038, ABclonal) at 4°C.

    Techniques: Cell Culture, Quantitative RT-PCR, Western Blot, Expressing, Immunofluorescence, Staining, Standard Deviation

    CAP regulates the PRKCQ/NF-κB axis The sh-PRKCQ- or sh-PRKCQ plus oe-PRKCQ-transfected iMC23 cells were treated with CAP for 60 s and then cultured for 48 h. (A) RT-qPCR analysis for evaluating PRKCQ knockdown or overexpression efficiency in iMC23 cells. (B) RT-qPCR analysis for PRKCQ expression in control, CAP(60s), CAP(60s) + sh-PRKCQ, and CAP(60s) + sh-PRKCQ + oe-PRKCQ groups. (C and D) Western blotting for detecting PRKCQ and p-NF-κB p65 expression. (E) CCK-8 assay for cell viability assessment. (F) Representative images of flow cytometry showing cell cycle distributions of iMC23 cells. (G) Quantitative results of cell cycle distributions. n = 3 biological replicates. Data are represented as mean ± standard deviation. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc analysis. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns, no difference.

    Journal: iScience

    Article Title: Cold atmospheric plasma (CAP) stimulates melanoblast-to-melanocyte differentiation via PRKCQ upregulation and NF-κB activation

    doi: 10.1016/j.isci.2026.115609

    Figure Lengend Snippet: CAP regulates the PRKCQ/NF-κB axis The sh-PRKCQ- or sh-PRKCQ plus oe-PRKCQ-transfected iMC23 cells were treated with CAP for 60 s and then cultured for 48 h. (A) RT-qPCR analysis for evaluating PRKCQ knockdown or overexpression efficiency in iMC23 cells. (B) RT-qPCR analysis for PRKCQ expression in control, CAP(60s), CAP(60s) + sh-PRKCQ, and CAP(60s) + sh-PRKCQ + oe-PRKCQ groups. (C and D) Western blotting for detecting PRKCQ and p-NF-κB p65 expression. (E) CCK-8 assay for cell viability assessment. (F) Representative images of flow cytometry showing cell cycle distributions of iMC23 cells. (G) Quantitative results of cell cycle distributions. n = 3 biological replicates. Data are represented as mean ± standard deviation. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc analysis. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns, no difference.

    Article Snippet: After blocking with 5% skim milk in 0.1% TBST, the membrane was subjected to overnight incubation with the following primary antibodies: anti-TRP-2 (ab221144, 1:1000, Abcam), anti-TYRP1 (ab235447, 1:1000, Abcam), anti-TYR (A1254, 1:500, ABclonal), anti-MITF (ab303530, 1:1000, Abcam), anti-protein kinase C Theta (PRKCQ; 1:1000, ab302891, Abcam), anti-p65 (1:5000, A19653, ABclonal), anti-p-p65 (1:500, AP0124, ABclonal), and anti-β-actin (1:80000, AC038, ABclonal) at 4°C.

    Techniques: Transfection, Cell Culture, Quantitative RT-PCR, Knockdown, Over Expression, Expressing, Control, Western Blot, CCK-8 Assay, Flow Cytometry, Standard Deviation